Técnicas de Laboratório em Biologia II

At the end of the semester it is expected that students have acquired the following skills: (1) Describe and explain the molecular basis associated with the several techniques discussed during the course. (2) Distinguish and describe the molecular biology methods that are used to isolate, purify and characterize nucleic acids (gel electrophoresis (agarose), restriction mapping, PCR, hybridization of nucleic acids and sequencing). (3) Compare and contrast the different approaches described throughout the course. Critically associate these differences to the desired end goal. (4) Regarding a specific biological question, identify the experimental approach most appropriate to address a specific problem. (5) Analyze and interpret experimental results, critically and clearly. Present the results in the form of a scientific report. (6) Understand the importance of technical evolution over the years and that the methodologies are technically limited to the existing knowledge. (7) Understand that molecular and cellular biology are areas in constantly development, always on the edge of the latest technical breakthroughs. (8) Acquire skills to subsequently attend Molecular Genetics and Genetic Engineering Course Units.